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:: Volume 6, Issue 19 (9-2018) ::
NPWJM 2018, 6(19): 54-59 Back to browse issues page
Chronic complications of sulfur mustard on endocrine glands ( liver and testis) based on histochemistry
Abstract:   (112 Views)
Introduction: Sulfur mustard is a gas warfare that is absorbed through inhalation and direct contact with the skin or through the digestive tract following contaminated food. In human studies, non-hydrolysed mustard in the brain and fat deposits even after many days after exposure. Disposal of this substance occurs through body secretions such as semen, urine, sweat and blood. The effect of mustard is mainly on the level of other toxic materials, ie, fast cell division, and liver cells.
Methods: In this research, 30 male albino 200g were divided into three groups: normal, solvent group , and sulfur mustard group. For the experimental group, 10 micrograms per kilogram of mustard dissolved in a tyrods buffer was injected intraperitoneally. After 8 weeks, the rats were anesthetized and then slaughtered and described in the hood and sampled from the liver and testis. The sample was placed in a 10% formalin for tissue processing and was applied to the microtome of 5 micron sections. The specimens were studied by light microscopy after hematoxylin-eosin staining. The results were analyzed using SPSS software and T-test. P <0.05 was considered statistically significant.
Results: In the mustard group, the liver tissue was solidified and dried, and extensive necrosis areas were observed in the lobules, as well as vacuolating hepatocytes, hepatocyte necrosis, edema of the port, and blood cell infiltration and lymphocytes. The thickness of germinal epithelium in the mustard group decreased, and the adluminal cells were isolated and discontinuous. The number of seminiferous tubule in the microscopic section was reduced in the mustard group and interstitial edema was seen, and leydig cells also became smaller in the mustard group.
Conclusion: The results of this study have shown that changes in the appearance of the liver, necrosis in the hepatocyte, as well as the destruction of the liver cells, dilatation and hypertension of the sinuses, the formation of fatty vacuoles in the hepatocyte cytoplasm, swelling and Picnotic hepatocytes as well as blood cell infiltration were observed in sinusoids, central vein of the lobule and port in the liver. In the testis, loss of the integrity of the testicular tissue, increased space between the seminiferous tubule and interstitial edema, reduced thickness of germ epithelium, and the presence of necrotic forms of spermatocyte cells and reduced its number, decreased the volume and number of Leydig cells. In conclusion, it can be concluded that Mustard can cause spermatogenesis in years after exposure, leading to infertility and changes in the level of liver enzymes and their function.
Keywords: Sulfur Mustard, Liver, Testis, Rat
Full-Text [PDF 650 kb]   (46 Downloads)    
Type of Study: Research | Subject: Special
Received: 2018/12/28 | Accepted: 2018/12/28 | Published: 2018/12/28
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Chronic complications of sulfur mustard on endocrine glands ( liver and testis) based on histochemistry. NPWJM. 2018; 6 (19) :54-59
URL: http://npwjm.ajaums.ac.ir/article-1-557-en.html


Volume 6, Issue 19 (9-2018) Back to browse issues page
پرستار و پزشک در رزم Nurse and Physician Within War
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