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The effect of cryotherapy on inflammation and volume of post-hypoxic lesions in male rat
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Abstract: (2669 Views) |
Introduction: hypoxia result in harmful changes in brain structure and function. Reduction of inflammation, inhibition of apoptosis, repair of damaged axons and angiogenesis are factors that can Helps repair nerve tissue in damaged areas and reduce complications.Cryotherapy reduces cellular damage by decreasing edema and complications caused by reperfusion.
Methods: In this study, 16 male Wistar rats ,weighing 250 g, were used in two groups include hypoxia and treatment. For anesthesia, chloral hydrate was used IP at a dose of 400 mg / kg.The left carotid artery was temporarily interrupted for one hour and the oxygen supply was disrupted. By restarting the bloodstream, the ice pack is applied locally to the rat's neck for half an hour. After 24 hours, the rats were sacrified and the samples were stained with cresyl violet. Then taken with Canon's digital camera.Cerebral edema, lesion volume and number of cell death were calculated and analyzed by SPSS software.
Results : Dead cells are reduced in the treatment group and normal cells are more likely to be seen. The use of topical cryotherapy has reduced brain edema. The volume of lesion in the treatment group was significantly lower than the hypoxia group.
Conclusion: The results of this study have shown that the use of topical cryotherapy reduces brain damage by reducing reperfusion complications. Using the results obtained from this study, it can be concluded that the use of topical cryotherapy has the same effect as drug that decrease in the body temperature but can produce a favorable effect without systemic effects in the body and its long-term use is harmful. In addition, it is easy to use and available and can be used as an initial treatment and at the same time as other treatments |
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| Keywords: Hypoxia, Ischemia, Brain, Cryotherapy, Rat |
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Full-Text [PDF 485 kb]
(1836 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2018/07/9 | Accepted: 2018/07/9 | Published: 2018/07/9
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