Introduction: this study has been prevalence and antibiotic resistance profiles of the clinically significant coagulase negative staphylococcal (CNS) isolates found in health care workers (HCWs) of a military hospital.

Methods: The cross-sectional study was carried out on 118 male and female HCWs during December 2014 to March 2015 in Kermanshah, Iran. Samples were taken from the anterior region nose of HCWs by Sterile cotton swabs and were cultured on Blood agar. KB004 Histaph Kit was performed for differentiation of Staphylococcus species.

Results: A total of 118 HCWs, 73 (62%) carried CNS and the mean age of the individuals was 27.56 years, 47(39.8%) were women and 71(60.2%) were men.  Among CNS isolates Staphylococcus epidermidis (63%) and saprophyticus (26.5%) showed the most frequency and CNS isolates were the most susceptibility to mupirocin and vancomycin.

Conclusion: HCWs act as a potential source of infection to their patients, causing nosocomial infection in the hospital. Our study suggests which can be used for control spread of CNS especially in hospitals are regular screening of the HCWs.