fa معتبرسازی روش آنالیز باقی مانده 25 آفت کش در برنج با استفاده از دستگاه LC-MS/MS (کروماتوگرافی مایع– طیف سنجی جرمی) تخصصي Special پژوهشي Research <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:107%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></span></b><b> </b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">در سال های اخیر به دلیل افزایش مصرف آفتکش ها در محصولات کشاورزی و به طبع ورود آن به زنجیره غدایی انسان موجب افزایش نگرانی هایی در مورد سلامت غذا، در بین مردم شده است.ازاین رو،هدف از انجام این پژوهش</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">معتبرسازی روش آنالیز باقی مانده 25&nbsp; آفت کش در برنج با استفاده از دستگاه</span></span></span> <span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">LC</span></span></span><span dir="LTR" style="font-size:14.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">-</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">MS/MS</span></span></span><span lang="FA" style="font-size:14.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">ست.</span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:107%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">روش کار</span></span></span></b><b><span lang="FA" style="font-size:14.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">در این پژوهش یک روش آنالیز همزمان برای تعیین مقدار 25 نوع آفتکش پر مصرف در برنج با استفاده از دستگاه کروماتوگرافی مایع با طیف سنج جرمی متوالی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">راه اندازی شد. مراحل آماده سازی نمونه ها بر اساس روش بهینه شده </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">QuEChERS</span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">، با استفاده از استخراج بافری و پاکسازی بر اساس متد </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">d-SPE</span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> انجام شد. تکنیک </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">LC</span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">-</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">MS/MS</span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> با یونیزاسیون الکترواسپری در مد مثبت برای تعیین همزمان 25 نوع آفتکش در یک اجرای 20</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">دقیقه ای و در برنامه </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">MRM</span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> بکار گرفته شد. </span></span></span><b><span lang="FA" style="font-size:14.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin""></span></span></span></b></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:107%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">نتایج:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">حد تعیین مقدار </span></span></span><span new="" roman="" style="font-family:" times="">(</span><span dir="LTR" new="" roman="" style="font-family:" times="">LOQ</span><span new="" roman="" style="font-family:" times="">)</span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> و حد تشخیص </span></span></span><span new="" roman="" style="font-family:" times="">(</span><span dir="LTR" new="" roman="" style="font-family:" times="">LOD</span><span new="" roman="" style="font-family:" times="">)</span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> برای تمامی آفتکش ها به ترتیب، 25 و 8 نانوگرم/گرم بدست آمد. 30 نمونه جمع آوری شده برنج وارداتی از سطح شهر تهران جمع آوری و آنالیز شدند. از تعداد کل 30 نمونه، 9 نمونه یعنی 30</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span arial="" style="font-family:">٪</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> نمونه ها به سموم </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Triazophos, Phoratesulfoxide, Fosthiazate, Formetanate</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">, </span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Fonofos, Cyphenothrin</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> آلودگی داشتند؛ که از این تعداد 4 نمونه بالاتر از </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">MRL</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> تعیین شده توسط سازمان استاندار ملی ایران بود<b>.</b></span></span></span><b><span lang="FA" style="font-size:14.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin""></span></span></span></b></span></span></span></span></span></span></span><br> &nbsp; <span class="HwtZe" jsaction="mouseup:Sxi9L,BR6jm; mousedown:qjlr0e" jsname="jqKxS" lang="en"><span class="jCAhz ChMk0b" jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb;bmeZHc:iURhpf;Oxj3Xe:qAKMYb,yaf12d" jscontroller="Gn4SMb" jsname="txFAF"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb">Introduction: In recent years, due to the increase in the use of pesticides in agricultural products and its entry into the human food chain, there has been an increase in concerns about the health of food among people. Therefore, the purpose of conducting this research is to validate the analysis method.</span></span> <span class="jCAhz ChMk0b" jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb;bmeZHc:iURhpf;Oxj3Xe:qAKMYb,yaf12d" jscontroller="Gn4SMb" jsname="txFAF"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb">25 pesticide residues in rice using LC-MS/MS.</span></span><span class="jCAhz" jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb;bmeZHc:iURhpf;Oxj3Xe:qAKMYb,yaf12d" jscontroller="Gn4SMb" jsname="txFAF"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb"> </span></span><span class="jCAhz ChMk0b" jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb;bmeZHc:iURhpf;Oxj3Xe:qAKMYb,yaf12d" jscontroller="Gn4SMb" jsname="txFAF"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb">Methods: In this research, a simultaneous analysis method was set up to determine the amount of 25 commonly used pesticides in rice using a liquid chromatography device with a sequential mass spectrometer.</span></span> <span class="jCAhz ChMk0b" jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb;bmeZHc:iURhpf;Oxj3Xe:qAKMYb,yaf12d" jscontroller="Gn4SMb" jsname="txFAF"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb">Sample preparation steps were performed based on QuEChERS optimized method, using buffer extraction and purification based on d-SPE method.</span></span> <span class="jCAhz ChMk0b" jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb;bmeZHc:iURhpf;Oxj3Xe:qAKMYb,yaf12d" jscontroller="Gn4SMb" jsname="txFAF"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb">LC-MS/MS technique with electrospray ionization in positive mode was used for the simultaneous determination of 25 types of pesticides in a 20-minute run and in the MRM program.</span></span><span class="jCAhz" jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb;bmeZHc:iURhpf;Oxj3Xe:qAKMYb,yaf12d" jscontroller="Gn4SMb" jsname="txFAF"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb"> </span></span><span class="jCAhz ChMk0b" jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb;bmeZHc:iURhpf;Oxj3Xe:qAKMYb,yaf12d" jscontroller="Gn4SMb" jsname="txFAF"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb">Results: The limit of quantification (LOQ) and limit of detection (LOD) for all pesticides were 25 and 8 ng/g, respectively.</span></span> <span class="jCAhz ChMk0b" jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb;bmeZHc:iURhpf;Oxj3Xe:qAKMYb,yaf12d" jscontroller="Gn4SMb" jsname="txFAF"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb">30 samples of rice imported from Tehran were collected and analyzed.</span></span> <span class="jCAhz ChMk0b" jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb;bmeZHc:iURhpf;Oxj3Xe:qAKMYb,yaf12d" jscontroller="Gn4SMb" jsname="txFAF"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb">Out of the total number of 30 samples, 9 samples, i.e. 30% of the samples, were contaminated with Triazophos, Phoratesulfoxide, Fosthiazate, Formetanate, Fonofos, and Cyphenothrin toxins;</span></span> <span class="jCAhz ChMk0b" jsaction="agoMJf:PFBcW;MZfLnc:P7O7bd;nt4Alf:pvnm0e,pfE8Hb,PFBcW;B01qod:dJXsye;H1e5u:iXtTIf;lYIUJf:hij5Wb;bmeZHc:iURhpf;Oxj3Xe:qAKMYb,yaf12d" jscontroller="Gn4SMb" jsname="txFAF"><span class="ryNqvb" jsaction="click:E6Tfl,GFf3ac,tMZCfe; contextmenu:Nqw7Te,QP7LD; mouseout:Nqw7Te; mouseover:E6Tfl,c2aHje" jsname="W297wb">Of these, 4 samples were higher than the MRL determined by the National Governor&#39;s Organization of Iran.</span></span></span><br> &nbsp; آفت کش, کروماتوگرافی مایع با طیف سنج جرمی, برنج, QuEChERS. Pesticide, Liquid Chromatography with Mass Spectrometry, Rice, QuEChERS. 25 33 http://npwjm.ajaums.ac.ir/browse.php?a_code=A-10-4370-1&slc_lang=fa&sid=1 Maryam Amir Ahmadi مریم امیر احمدی maryamghanbarinejad@gmail.com 10031947532846005604 10031947532846005604 No Food and Drug Organization سازمان غذا و دارو hamed Hassanvand حامد حسنوند hamedhasanvand70@yahoo.com 10031947532846005605 10031947532846005605 Yes Islamic Azad university دانشگاه آزاد اسلامی vahid hadi وحید هادی vahidhadi1@gmail.com 10031947532846005606 10031947532846005606 No Aja University of Medical Sciences دانشگاه علوم پزشکی اجا