Nurse and Physician within War
پرستار و پزشک در رزم
NPWJM
Medical Sciences
http://npwjm.ajaums.ac.ir
1
admin
2645-3940
2645-3932
8
10.52547/npwjm
fa
jalali
1400
12
1
gregorian
2022
3
1
9
33
online
1
fulltext
fa
تأثیر 8 هفته تمرین استقامتی شدید و تناوبی شدید بر میزان آنزیم تیوردوکسین ردوکتاز-یک و مالون دی آلدهید در بافت ریه موش های صحرایی نر ویستار
Effects of 8 Week Strenuous Endurance and High Intensity Interval Training on Thioredoxin Reductase-1 enzyme and Malondialdehyde in Lung Tissue of Male Wistar rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">مقدمه: </span></span></span></b><span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">فعالیتهای جسمانی در هر شدت و مدتی بر روی فشار اکسایشی و سیستم ضد اکسایشی موثرند. لذا انتخاب مناسب نوع، زمان و شدت تمرین ضروری به نظر میرسد. بنابراین هدف از پژوهش حاضر بررسی اثر 8 هفته تمرین</span></span></span> <span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">استقامتی</span></span></span> <span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">شدید</span></span></span> <span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">و</span></span></span> <span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">تناوبی شدید</span></span></span> <span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">بر</span></span></span> <span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">تیوردوکسین ردوکتاز-1 و</span></span></span> <span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">مالو­ن </span></span></span><span dir="LTR" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif">­</span></span></span><span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">دی </span></span></span><span dir="LTR" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif">­</span></span></span><span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">آلدهید</span></span></span> <span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">در</span></span></span> <span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">بافت</span></span></span> <span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">ریه</span></span></span> <span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">موش­های</span></span></span> <span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">صحرایی</span></span></span><span dir="LTR" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif">­ </span></span></span><span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">نر</span></span></span> <span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">ویستار است.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">روشکار:</span></span></span></b><span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> 24 سر موش نر ویستار، به صورت تصادفی به 3 گروه (کنترل، تمرین استقامتی شدید، تمرین تناوبی شدید) تقسیم شدند. پروتکل تمرین استقامتی شامل دویدن بر روی نوار گردان به مدت 8 هفته (5 جلسه در هفته) و پروتکل تمرین تناوبی نیز شامل 8 هفته (5 جلسه در هفته) دویدن تناوبی بر روی نوار گردان بود. 48 ساعت پس از آخرین جلسه تمرینی موشها قربانی شدند و تحت شرایط استریل بافت ریه جدا شد. غلظت بافتی تیوردوکسین­ ردوکتاز-1 به روش الایزا و غلظت مالون­دی­آلدهید</span></span></span><span lang="AR-SA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> با روش تریباربیتوریک اسید</span></span></span> <span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">سنجش شد. برای تجزیه و تحلیل دادهها از آزمون واریانس یک طرفه با آزمون تعقیبی توکی استفاده شد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">یافتهها:</span></span></span></b><span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> پس از 8 هفته در میزان تیوردوکسین­ ردوکتاز-1 بافت ریه در گروههای تمرین استقامتی شدید و تناوبی شدید نسبت به گروه کنترل تغییر معنی­داری ایجاد نشد (0/43=</span></span></span><span dir="LTR" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif">p</span></span></span><span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">). همچنین میزان مالون دی آلدهید بافت ریه گروه تناوبی شدید نسبت به گروه کنترل تغییر معنی­داری نداشت (0/06=</span></span></span><span dir="LTR" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif">p</span></span></span><span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">)، اما در گروه استقامتی شدید نسبت به گروه کنترل به طورمعنی­داری کاهش یافت (0/02=</span></span></span><span dir="LTR" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif">p</span></span></span><span lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">). </span></span></span></span></span></span></span></span><br>
<b><span dir="RTL" lang="FA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">نتیجهگیری: </span></span></span></b><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">با توجه به نتایج این پژوهش شاید بتوان گفت تمرینات استقامتی شدید 8 هفتهای ضمن کاهش مالون دی آلدهید، تاثیری بر میزان تیوردوکسین ردوکتاز-1 نداشته است.</span></span></span>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"><span style="letter-spacing:-.2pt">Introduction:</span></span></span></b> <span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">Physical activity of any intensity and time effect on the oxidative stress and Antioxidant system. So, the proper choice of type, time and intensity of exercise is necessary. Therefore, the aim of the present study was to investigate the effect of 8 weeks of strenuous</span></span> <span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">endurance (SET) and</span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"> high intensity</span></span><span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"> interval training (HIIT) </span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">on the </span></span><span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">TRX1</span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"> and MDA of lung tissue in male wistar rats.</span></span><span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">Methods</span></span></b><b><span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">:</span></span></b><span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"> 24 male Wistar rats, were randomly divided into three groups: (Control, SET, HIIT). The training protocols were consisted SET and HIIT on a treadmill for 8 weeks (5 days a week). Forty-eight hours after the last session of training lung tissue samples were collected. Lung concentration of TRX1 was measured by ELISA and MDA concentration was measured by </span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">tribarbituric acid. To analyze the data, one-way ANOVA and Tukey's post hoc test were used.</span></span><span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">Results: </span></span></b><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">The results of this study showed that after 8 weeks, </span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">there were no significant differences in </span></span><span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">TRX1</span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"> level </span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">of </span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">lung </span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">tissue in</span></span> <span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">SET and HIIT</span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"> groups compared to control group </span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">(p = 0/43).</span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"> Also, there were no significant differences in </span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">MDA level </span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">of </span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">lung </span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">tissue in</span></span> <span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">HIIT</span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"> groups compared to control group</span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"> (P = 0/06), but</span></span> <span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">the level of MDA </span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">was significantly decreased in </span></span><span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">SET</span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"> group compared to endurance group </span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">(P= 0/02).</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">Conclusion:</span></span></b><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"> According to the results of this study, it can be concluded that 8-week </span></span><span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">SET</span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">, while reducing MDA, did not on </span></span><span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">TRX1</span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"> level </span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">of </span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">lung </span></span><span lang="EN" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">tissue</span></span><span dir="RTL" lang="FA" style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif">.</span></span><b><span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"></span></span></b></span></span></span></span><br>
تمرین استقامتی شدید, تمرین تناوبی شدید, تیوردوکسین ردوکتاز -یک, مالون دی آلدهید, ریه
Endurance Training, Interval training, Thioredoxin reductase-1, Malondialdehyde, Lung
26
36
http://npwjm.ajaums.ac.ir/browse.php?a_code=A-10-298-4&slc_lang=fa&sid=1
aghaali
ghasemnian
آقاعلی
قاسم نیان
ghasemnian@znu.ac.ir
10031947532846005059
10031947532846005059
Yes
Department of Sport Sciences, Faculty of Humanities, University of Zanjan, Zanjan , Iran
گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه زنجان، دانشگاه زنجان، ایران
fariba
sojasi
فریبا
سجاسی
fariba.sijasi.@gmail.com
10031947532846005060
10031947532846005060
No
Department of Sport Sciences, Faculty of Humanities, University of Zanjan, Zanjan , Iran
گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه زنجان، دانشگاه زنجان، ایران
akram
, karimiasl Akram
اکرم
کریمی اصل
akramkarimi.a.sl@gmail.com
10031947532846005061
10031947532846005061
No
Department of Sport Sciences, Faculty of Humanities, University of Zanjan, Zanjan , Iran
گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه زنجان، دانشگاه زنجان، ایران
hamidreza
norouzi
حمیدرضا
نوروزی
h.norouzi@znu.ac.ir
10031947532846005062
10031947532846005062
No
Department of Sport Sciences, Faculty of Humanities, University of Zanjan, Zanjan , Iran
گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه زنجان، دانشگاه زنجان، ایران