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Evaluation of the effect of manganese oxide nanoparticles on Toxoplasma gondii in vitro
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Ali Dalir Ghaffari   , Amir KarimiPourSaryazdi , Pooya Tavakoli , Mohammad Barati , Yeganeh KarimiPourSaryazdi |
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Abstract: (2017 Views) |
Introduction: Pyrimethamine and sulfadiazine are selective medications for toxoplasmosis, but some side effects hinder their consumption. Increasing the use of nanoparticles in biological studies and showing the beneficial effects of manganese nanoparticles on fungi and bacteria, as well as the lack of sufficient knowledge on its anti-Toxoplasma impacts, was the motivation for the design of this study. Manganese can provoke cell apoptosis by increasing the activation of the FRXO3a-Bim/PUMA mRNA and caspase-3 pathway. The objective of this study was to investigate the efficacy of manganese oxide nanoparticles (Mn2O3 NPs) against Toxoplasma gondii (T. gondii) in vitro.
Methods: To assess the anti-Toxoplasma activity of Mn2O3 NPs, the light microscopic observation was applied to evaluate the number of residual parasites in each well. Then, the MTT method was used to specify the toxic effect of Mn2O3 NPs on T. gondii toxicity. Finally, the potential apoptosis of T. gondii by Mn2O3 NPs was investigated by flow cytometry assay
Result: The IC50 value of Mn2O3 NPs against T. gondii tachyzoite was 105 μg/ml. There was also no significant toxic effect of Mn2O3 NPs on macrophages due to the high percentage of surviving macrophages at the desired concentration for treatment. The findings of the flow cytometry revealed that about 40% of tachyzoites were caused to apoptosis with Mn2O3 NPs.
Conclusion: Mn2O3 NPs have a beneficial effect on T. gondii tachyzoite in vitro and could be regarded as a candidate for the treatment of this infection. |
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| Keywords: Toxoplasma gondii, Mn2O3 NPs, MTT, In vitro |
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Full-Text [PDF 641 kb]
(428 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2020/04/21 | Accepted: 2020/07/13 | Published: 2021/08/21
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